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| Figs. 1 - 6. One- and two-year-old peach stems inoculated with L. persoonii.
Figs. 1,5,6. Stereomicrographs. Figs. 2 - 4. Light micrographs. Fig. 1. Longitudinal
section through pith (P), xylem (X), and bark (B) (tissues are between opposite oriented
arrows), sampled seven days after inoculation with Leucostoma persoonii,
showing four polysaccharide-rich reaction zones (large arrows). Colonization from left to
right had proceeded in the bark through the first zone and was almost to the second. x15.
Fig. 2. Longitudinal section of peach bark and vascular cambial (VC) tissues seven days
after inoculation with L. persoonii showing hyphal aggregations (H) in
periderm and cortex regions and alteration of cambial derivatives (arrow). Colonization
proceeding from right to left. x420. Fig. 3. Longitudinal section of peach bark and
vascular cambial (VC) tissues seven days after inoculation with L. persoonii
showing hyphal aggregation (H) in cortex and alteration of cambial derivatives (arrow).
Colonization proceeding from right to left. Note that very little new phloem has been
produced. x560. Fig. 4. Inter- and intracellular colonization of phloem tissues by hyphae
of L. persoonii (arrows). Colonization proceeding from bottom to top of
micrograph. x680. Figs. 5,6. Transverse views of peach stems inoculated with L. persoonii
(Fig. 5) or noninfested malt agar (Fig. 6). Stems were excised at the base and immersed in
stain solution to demonstrate the location of nonfunctional xylem tissues (light areas,
NX). Note location of functional xylem (FX) in the new callus tissue in Fig. 6 versus the
regions of nonfunctional xylem in callus tissues in Fig. 5. Pith (P), gum ducts (GD), and
xylem barrier zone (BZ) are present. x8. (Fig. 1 from Biggs, 1984). |
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