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| Plate is an image map - click to enlarge individual photos. Figs.
2.3-2.7. Light micrographs of longitudinal sections through tissue undergoing
dedifferentiation during generation of new phellogen after mechanical wounding of bark of
healthy 2-year-old peach bark. Outside bark in all figures is oriented toward top of
plate. Bar = 10 m. Fig. 2.3. Section through the secondary phloem 48 hr postwounding
showing mitotic activity associated with tissue redifferentiation. Cells to the left of
the arrows participate in phellogen generation; cells to the right are becoming
hypertrophied and developing lignified walls and intracellular suberin linings. Fig. 2.4.
Section through periderm and cortex 72 hr postwounding showing two distinct tissue regions
in the transition zone between healthy (left) and necrotic tissue (right). Tissue to the
right of the arrows shows first positive reaction with phloroglucinol + HCl but is not yet
impervious to fluid diffusion. Fig. 2.5. Section of differentiated phellogen (PG) and
incipient impervious tissue (IT and arrows) 96 hr postwounding. Figs. 2.6 and 2.7. Bark
tissue stained with phosphine, under bright field and the same under ultraviolet
excitation, respectively, showing impervious tissue (IT), first phellem (PH), and newly
regenerated phellogen (PG) 7 days postwounding. Note in Fig. 2.7 the thin suberin lining
of the cells in the impervious zone (small arrows). |
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