Project Number: WVA00413
CRIS Number: 0185011
BIOCHEMICAL AND MOLECULAR ASPECTS OF LYSINE DEGRADATION
Investigators: Blemings, K. P.
Performing Department: Animal & Veterinary Science -- 1877
Start Date: 04/01/2000
Termination Date: 03/31/2005
Reporting period: 01/01/2001 to 12/31/2001
Progress Report:
This past year we have determined the in-vitro oxidation of lysine in different tissues using both uniformly and one-labeled lysine. Data indicate that the oxidation of lysine is more complete in liver than in muscle or intestine. This finding is consistent with the possibility that the different tissues oxidize lysine by different pathways. We have begun to develop an RT-PCR approach to determine the presence of the lysine alpha-ketoglutarate reductase mRNA in different tissues. This will strengthen our conclusion about the relative importance of the lysine alpha-ketoglutarate reductase-dependent pathway in different tissues. We have also begun to purify the lysine alpha-ketoglutarate reducatse protein to that we can generate antibodies for different immunodetection protocols.
Publications:
Manangi, M.K., Hoewing, S.F.A., Engels, J.G., Blemings, K.P. 2001. Extra-hepatic tissues contribute to lysine oxidation in chicks. FASEB J. A268.
Impact:
This work will allow us to determine the relative importance and contribution of the different pathways of lysine oxidation to whole-body lysine catabolism.